Journal: ACS Omega

Article Title: Fragment Libraries from Large and Novel Synthetic Compounds and Natural Products: A Comparative Chemoinformatic Analysis

doi: 10.1021/acsomega.5c01420

Figure Lengend Snippet: Natural Product Libraries Studied in This Work

Article Snippet: The goal of this study was to analyze the contents, properties, and chemical diversity of fragment libraries obtained from two large natural product libraries (COCONUT 2.0 and the most updated version of LANaPDB), chemical synthesis (CRAFT), and commercial fragment libraries (Enamine, ChemDiv, Maybridge, and Life Chemicals).

Techniques:

Journal: Journal of Natural Medicines

Article Title: Resveratrol as a BCL6 natural inhibitor suppresses germinal center derived Non-Hodgkin lymphoma cells growth

doi: 10.1007/s11418-024-01873-4

Figure Lengend Snippet: Resveratrol is identified as a BCL6 BTB natural inhibitor. A Schematic diagram of HTRF assay screening for BCL6 inhibitors. B Chemical structure of resveratrol. C Resveratrol and FX1 were tested in HTRF assay for inhibition of the BCL6-SMRT interaction. (D) Schematic diagram of dual-luciferase reporter assay. E Luciferase reporter assay was performed to test the activity of resveratrol against the repressor activity of BCL6 BTB . When plasmids (GAL 4 ) 5 -TK-LUC combined with GAL 4 -DBD-BCL6 BTB , the fluorescence expression was inhibited. After adding 100 μM of resveratrol, the fluorescence was restored. F K D of resveratrol binding to BCL6 BTB protein determined by an SPR assay. G Molecular docking model predicted that resveratrol bound to the BCL6-BTB domain. H The binding affinities of resveratrol and BCL6 mutants

Article Snippet: The natural product library was sourced from Targetmol (L6700), and resveratrol (purity 99.9%) was purchased from Targetmol (T1558, Boston, MA).

Techniques: HTRF Assay, Screening, Inhibition, Luciferase, Reporter Assay, Activity Assay, Fluorescence, Expressing, Binding Assay, SPR Assay, Docking Assay, Förster Resonance Energy Transfer

Journal: Journal of Natural Medicines

Article Title: Resveratrol as a BCL6 natural inhibitor suppresses germinal center derived Non-Hodgkin lymphoma cells growth

doi: 10.1007/s11418-024-01873-4

Figure Lengend Snippet: Resveratrol induces de-repression of BCL6 target genes p53, ATR, CXCR4, CDKN1A, CD69 and CD80. (*, P < 0.05; **, P < 0.01; ***, P < 0.001 versus control)

Article Snippet: The natural product library was sourced from Targetmol (L6700), and resveratrol (purity 99.9%) was purchased from Targetmol (T1558, Boston, MA).

Techniques: Control

Journal: Journal of Natural Medicines

Article Title: Resveratrol as a BCL6 natural inhibitor suppresses germinal center derived Non-Hodgkin lymphoma cells growth

doi: 10.1007/s11418-024-01873-4

Figure Lengend Snippet: Resveratrol blocks GC formation in vivo. C57BL/6 mice were immunized with NP 18 -CGG and treated with resveratrol at a dosage of 100 mg/kg/d. A Flow cytometry analysis revealed that the proportion of GC B cells in the resveratrol group decreased. B Percentage of Tfh cells was reduced in the resveratrol group by flow cytometry. C Resveratrol affected class-switch recombination in flow cytometry analysis. D Resveratrol decreased the titers of the NP-specific immunoglobulin G1 in serum measured by ELISA with NP 5 -BSA and NP 23 -BSA. (*, P < 0.05; **, P < 0.01 versus control)

Article Snippet: The natural product library was sourced from Targetmol (L6700), and resveratrol (purity 99.9%) was purchased from Targetmol (T1558, Boston, MA).

Techniques: In Vivo, Flow Cytometry, Analysis, Enzyme-linked Immunosorbent Assay, Control, FACS

Journal: Journal of Natural Medicines

Article Title: Resveratrol as a BCL6 natural inhibitor suppresses germinal center derived Non-Hodgkin lymphoma cells growth

doi: 10.1007/s11418-024-01873-4

Figure Lengend Snippet: Resveratrol inhibits GC-derived NHL cells growth in vitro. A Anti-proliferative effects of resveratrol on GC-derived NHL cell lines and normal cell lines after 72 h of treatment. B IC 50 values of resveratrol in lymphoma cell lines and normal cell lines after 72 h of treatment. C – D Resveratrol synergized with EZH2 inhibitor GSK343 or PRMT5 inhibitor GSK591 to inhibit SUDHL4 cells growth. The combination indexes (CIs) are shown at the indicated concentrations

Article Snippet: The natural product library was sourced from Targetmol (L6700), and resveratrol (purity 99.9%) was purchased from Targetmol (T1558, Boston, MA).

Techniques: Derivative Assay, In Vitro

Journal: Journal of Natural Medicines

Article Title: Resveratrol as a BCL6 natural inhibitor suppresses germinal center derived Non-Hodgkin lymphoma cells growth

doi: 10.1007/s11418-024-01873-4

Figure Lengend Snippet: Resveratrol suppresses BCL6-driven lymphoma growth in vivo. A SUDHL4 xenograft mouse model. B Antitumor effects of resveratrol on SUDHL4 xenograft mouse model. Tumor volumes evaluated once every 3 days for a total of 15 days. C Tumor burden was weighed. D The body weight of the mice was measured every 3 days. E mRNA expression of the BCL6 target genes p53, ATR, CXCR4, CDKN1A, CD69 and CD80 from tumors was measured by RT-qPCR assays. (*, P < 0.05; **, P < 0.01; ***, P < 0.001 versus control)

Article Snippet: The natural product library was sourced from Targetmol (L6700), and resveratrol (purity 99.9%) was purchased from Targetmol (T1558, Boston, MA).

Techniques: In Vivo, Xenograft Assay, Expressing, Quantitative RT-PCR, Control